ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanisms underlying in the treatment of inflammatory bowel disease by Pulsatilla Decoction.</p><p><b>METHODS</b>Forty Wistar male rats were randomly divided into 5 groups( n = 8)control group, model group, model + positive control group (mesalazine), Pulsatilla Decoction treatment group, in addition, the Pulsatilla Decoction treatment group was divided into middle and high dose group. Intragastric administration was used in the positive control group and Pulsatilla Decoction treatment group. The expression of interleukin-1beta (IL-1beta), interleukin-6(IL-6) and tumor necrosis factor-alpha (TNF-alpha) were detected by real time PCR after extraction of RNA from colons.</p><p><b>RESULTS</b>Compared with the model group, positive medicine and Pulsatilla Decoction group, especially high-dose group, could effectively inhibit the expression of IL-1beta, IL-6 and TNF-alpha.</p><p><b>CONCLUSION</b>Pulsatilla Decoction could exert its effect in the treatment of inflammatory bowel disease by inhibiting the expression of proinflammatory cytokines.</p>
Subject(s)
Animals , Male , Rats , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Inflammatory Bowel Diseases , Drug Therapy , Metabolism , Interleukin-1beta , Genetics , Metabolism , Interleukin-6 , Genetics , Metabolism , Phytotherapy , Pulsatilla , Chemistry , RNA, Messenger , Genetics , Metabolism , Rats, Wistar , Tumor Necrosis Factor-alpha , Genetics , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate molecular mechanisms underlying in the treatment of inflammatory bowel disease by Pulsatilla decoction.</p><p><b>METHODS</b>Wistar male rats were randomly divided into control group, model group, model + positive control group (mesalazine), traditional Chinese medicine treatment group, in addition, the Chinese medical treatment group was divided into middle and high dose group ( n = 8). Intragastric administration was used in the positive control group and traditional Chinese medicine treatment group. The expression of Smad7 and p-Smad3 in the colons were detected by immunohistochemistry and Western blot.</p><p><b>RESULTS</b>Compared with the model group, positive medicine and traditional Chinese medicine group, especially high-dose group, could effectively inhibit the expression of Smad7, while enhancing the p-Smad3 expression.</p><p><b>CONCLUSION</b>The activation of TGF-beta1/Smad3 signaling pathway may be the molecular mechanism underlying in the anti-inflammatory effect of inflammatory bowel disease by Pulsatilla decoction.</p>